Benassi, L., G. Bertazzoni, C. Magnoni, A. di Nardo, L. Grenzi, and S. Seidenari. Effects of Tensides on Normal Human Keratinocytes: A Decrease in Toxic Potential When Used in Association. ATLA 1999. 27: 342.

Tegobetaine – 52504-27-5; Tween 20 – 09005-64-5; Tween 80 – 09005-65-6; sodium lauryl sulfate – 00151-21-3

Sodium lauryl sulfate (SLS) is an anionic tenside that is widely utilized as a model for studying acute and cumulative irritation. When used in culture, SLS induces an inhibition of DNA synthesis and a decrease in cellular viability. It has previously been reported that the barrier damage caused by SLS in vivo is lower when SLS is applied to the skin in combination with other tensides. The aim of our study was to evaluate whether the cytotoxic effect of SLS is reduced by the association with various tensides in vitro. Normal human keratinocytes from plastic surgery were grown in serum free medium. At subconfluency cells were treated with SLS at a dose corresponding to the effective dose 50%, in combination with Tween 20, Tween 80, Tegobetaine F50 at the minimum toxic dose. Following tenside treatment, the culture medium was changed and after 24 hours the cells were collected for thymidine incorporation, MTT assay and neutral red uptake. The cytotoxic effect on normal human keratinocytes as evaluated by thymidine incorporation, MTT assay and neutral red uptake was significantly decreased by combination with all the tested tensides.