Benassi, L., G. Bertazzoni, C. Magnoni, A. Di Nardo, E. Euclidi and S. Seidenari. Sodium Lauryl Sulphate Causes Interleukin-1a Release from Normal Human keratinocytes: Dose-Response and Time-Course Curves. ATLA 1999. 27: 343.

 

Sodium lauryl sulfate � 00151-21-3

 

Interleukin-1a is involved in the biological response to irritants as one of the most important inflammatory mediators.� The aim of the present study was to evaluate IL-1a production after exposure of cultured human keratinocytes to sodium lauryl sulphate (SLS), representing a model which enables the assessment of the in vitro effects of SLS on epidermal cells independently from the skin immune system and barrier alterations.� Normal human keratinocytes from plastic surgery were grown in serum free medium.� At subconfluency, cells were treated with SLS doses ranging from 0.00001% to 0.005%, and after exposure for one hour, the medium was changed.� At different time-points, the supernatant was collected for ELISA, and cells were harvested for Western blot analysis of pro-IL-1a and IL-1a.� Extracellular secretion of I-1a from keratinocytes was increased in a dose-dependent manner following SLS treatment.� The release of IL-1a starts 30 minutes after exposure to SLS (effective dose 50%) reaching a maximum at 3 hours.� Western blot analysis showed a down regulation of pro-IL-1a levels, while IL-1a levels remained unchanged.�